Skip to main content
. 2020 Aug 1;2020:4074832. doi: 10.1155/2020/4074832

Figure 5.

Figure 5

SCFA-mediated antioxidant and anti-inflammatory effects were partly reversed by siRNA-GPR43. qRT-PCR was performed to detect GPR43 and GPR41 mRNA levels. (b) Western blotting-based assay for the expression of GPR43 and MCP-1 after a 30 mM high glucose challenge for 6, 12, and 24 h. (c) The effects of indicated concentrations of SCFAs or a GPR43 agonist on GPR43 and MCP-1 expression were analyzed by western blotting. Following 30 mM high glucose for 24 h, SCFAs or a GPR43 agonist-ameliorated ROS (d), MDA (e), NF-κB signal (g), and MCP-1 (f) were significantly reversed by siRNA-GPR43. Ac: acetate; Pr: propionate; But: butyrate; ∗p < 0.05 compared with the NC group; #p < 0.05 compared with the HG group. &p < 0.05 compared with the Ac or Pr group.