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. 2020 Aug 10;4(15):3728–3740. doi: 10.1182/bloodadvances.2020002326

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Figure 1. — Correlation of phenotype and genotype in the dual-age–specific leukemia mouse model. (A) Kaplan-Meier survival curves show the relationship between survival time and genotype (P < .001). Death was counted in mice without any intervention. Male/female ratios were 2:1 in PtenΔ/ΔNf1^LOH mice and Pten^+/ΔNf1^LOH mice and 1:1 in PtenΔ/ΔNf1^+/Δ mice and WT mice. (B) Relationship between the genotype and the spleen and liver weights. Organs from littermates were collected at PND17-19 when PtenΔ/ΔNf1^LOH mice with JMML were moribund. Data are presented as median ratios of spleen or liver weight/body weight (BW). (C) Representative photomicrographs of hematoxylin and eosin–stained tissue sections from PtenΔ/ΔNf1^LOH mice with JMML (right panels) and WT control littermates (left panels) at PND18. Infiltrates are indicated by yellow arrows. (D-I) Blood profiles from juvenile PtenΔ/ΔNf1^LOH mice and littermates at PND17-19 when diseased mice were moribund (n = 12-21). Complete blood counts were performed with a Vet Abc Hematological analyzer. Differentials were manually counted from blood smears stained with May-Grünwald-Giemsa. (J-O) Flow cytometry analysis of cell subpopulations in BM, PB, and spleen. Data are mean ± standard error. *P < .05, ** P <.01, ***P < .001. See additional supportive data in supplemental Figures 1-7. Granu, granulocytes; HGB, hemoglobin; Lymph, lymphocytes; Mon, monocytes; PLT, platelets; RBC, red blood cells.

Figure 1. — Correlation of phenotype and genotype in the dual-age–specific leukemia mouse model. (A) Kaplan-Meier survival curves show the relationship between survival time and genotype (P < .001). Death was counted in mice without any intervention. Male/female ratios were 2:1 in PtenΔ/ΔNf1^LOH mice and Pten^+/ΔNf1^LOH mice and 1:1 in PtenΔ/ΔNf1^+/Δ mice and WT mice. (B) Relationship between the genotype and the spleen and liver weights. Organs from littermates were collected at PND17-19 when PtenΔ/ΔNf1^LOH mice with JMML were moribund. Data are presented as median ratios of spleen or liver weight/body weight (BW). (C) Representative photomicrographs of hematoxylin and eosin–stained tissue sections from PtenΔ/ΔNf1^LOH mice with JMML (right panels) and WT control littermates (left panels) at PND18. Infiltrates are indicated by yellow arrows. (D-I) Blood profiles from juvenile PtenΔ/ΔNf1^LOH mice and littermates at PND17-19 when diseased mice were moribund (n = 12-21). Complete blood counts were performed with a Vet Abc Hematological analyzer. Differentials were manually counted from blood smears stained with May-Grünwald-Giemsa. (J-O) Flow cytometry analysis of cell subpopulations in BM, PB, and spleen. Data are mean ± standard error. *P < .05, ** P <.01, ***P < .001. See additional supportive data in supplemental Figures 1-7. Granu, granulocytes; HGB, hemoglobin; Lymph, lymphocytes; Mon, monocytes; PLT, platelets; RBC, red blood cells.