Figure 6.

Dysregulated signal transduction pathways and molecular defects in mice with Pten deletion, Nf1 deficiency, and JMML. (A) Representative data from western blot analyses of the elements in GM-CSF signal transduction pathways in unfractionated BM nucleated cells from juvenile mice with various copy numbers of Pten and Nf1. Representative data were from ≥3 experiments from littermate mice at PND17-19. (B) Gene ontology (GO) analysis. Genes that were significantly over- or underexpressed in LIN⁻ BM cells from mice with JMML and WT littermates at age PND17 were used in GO analysis, together with HCA. (C) Heatmap and HCA of 182 genes that were differentially expressed in this study (PtenΔ/Δ Nf1^LOH/WT) vs the data (Fetal Liver/Adult BM) reported by Manesia et al.³⁴ We found a log₂-fold change in either being positive or negative consistently in both studies (182 genes). See additional supportive data in supplemental Figures 13 and 14 and supplemental Tables 4 and 5 for “GO HeatMap Terms & GeneIDs” and “Combined Analysis DEG Results.”