Table 2.

Primers and reaction conditions for mitochondrial DNA fragment amplification

No.	Primer	Sequence (5′-3′)	HC_F		SI_C		Annealing temperature	Extension time
			Position (bp)	Identity (%)	Position (bp)	Identity (%)
I	HSM1F	TAACGGATTAAAGCACAGCACTGAA	9879-9903	96.00	9877-9901	100.00	61 °C	3 min 30 s
	HSM1R	CGCATAGAGCTTGAAGGGAATTTAA	13,094-13,118	96.00	13,089-13,113	100.00
II	HSM2F	TCTTGTTTTCTTGTTTTTGTGAGTT	2812-2836	68.00	12,874-12,898	100.00	54 °C	3 min
	HSM2R	CTCGTGTATCAACATCCATTCC	3541-3562	63.64	886–907	95.45
III	HSM3F	TGCCATGATTGCAATAGGAGT	825–845	85.71	825–845	100.00	57 °C	3 min 30 s
	HSM3R	ATCTACAAAGTGTCAGTATCAGGCA	4251-4275	92.00	4250-4274	100.00
IV	HSM4F	CCACTTCTCAACCCATCACCACTTT	4212-4236	92.00	4211-4235	96.00	53 °C	3 min 30 s
	HSM4R	CTATTCCTTGGGGGCCTATTTCTTC	8060-8084	80.00	8058-8082	100.00
V	HSM5F	TTTTATCTCCTCCCTTTTTATYTCT	8008-8032	76.00	8006-8030	96.00	55 °C	2 min
	HSM5R	CCTCWAAAGTAGTTAAGATTGGGAC	9955-9979	76.00	9953-9977	96.00
VI	HcM1F	ATCCTGCCTTCCGTTATTTTA	9879-9903	96.00	9877–9901	100.00	53 °C	2 min
	HcM1R	CAACAGTGGTTTGGTCCTTCT	13,094-13,118	96.00	13,089-13,113	100.00
VII	RandomF	CCGCAAGGGAAAGATGAAATAC	14,289-14,310	100.00	14,285-14,306	100.00	52 °C	4 min
	RandomR	GGGGTGTTATTCTTCTAAGTATTGA	2600-2624	84.00	2598-2622	100.00
VIII	HcM2F	GAAGGACAAGAACTGGAGAC	2096-2116	100.00	1698-1717	75.00	53 °C	3 min
	HcM2R	CTTTGCGAGATAGATTTAGC	4851–4870	100.00	10,672-10,691	33.33
IX	HcM3F	ACTTTTGTTTTTCAATAAATCCCTCCA	7680-7706	100.00	8631-8657	70.37	55 °C	2 min 30 s
	HcM3R	TTTCTTTCTAACCACCCTTTTCACC	10,229-10,253	100.00	10,230-10,254	88.00
X	RDF	TATCATTTAGTAGACCAAAGCCCAT	3559-3583	100.00	3557-3582	100.00	52 °C	40 s
	RDR	CCTGTAGCGACAAAGAAGGTAGAAC	4118-4142	80.00	4117-4141	100.00