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‘Scar-in-a-jar’ HCS assay metrics and compound potency for standard inhibitors
| pIC50 | Assay metrics | |||||
|---|---|---|---|---|---|---|
| MEAN | STDEV | %CV | Assay window | n | Z prime | |
| PGE2 | 7.65 | 0.98 | 12.7 | 4.64 | 8 | 0.50 |
| SB-525334 | 6.79 | 0.33 | 4.8 | 4.63 | 28 | 0.49 |
| CZ415 | 6.38 | 0.31 | 4.8 | 4.67 | 25 | 0.51 |
Average pIC50, standard deviation (STDEV), and % coefficient of variance (%CV) values of the assay controls: SB-525334, PGE2 and CZ415 and assay parameters to measure assay performance and robustness
