Fig. 4.

Western blot analysis showing AKAP4, ODF1, ODF2, GAPDHS, SPESP1, and ACTRT2 proteins from control and heat treatment sperm of human and mouse. a Western blot analysis showing AKAP4, ODF1, ODF2, GAPDHS, SPESP1, and ACTRT2 proteins from human sperms of control and heat treatment. GAPDH was used as a loading control. b Quantification of the expression of the proteins shows significantly decreased levels in heat treatment groups. Data are presented as mean ± SEM, n = 3. *, P < 0.05. c-d Western blot analysis showing AKAP4, ODF1, ODF2, GAPDHS, SPESP1, and ACTRT2 proteins expression from mouse sperms of control and heat treatment (c) and the quantification of these proteins levels in (d). Data are presented as mean ± SEM, n = 3. *, P < 0.05