Table 2.
EWAS and/or GWAS (within 200 kb of CpG) replication of 48 differentially methylated CpG sites
| CpG_ID | Chromosome:Position | Gene | Gene_Group | Obesity(-related) associations in EWAS catalog (p<1×10–3) | Obesity(-related) association in EWAS Atlas (excluded p=NR) | Obesity(-related) association in GWAS catalog (within 200 Kb of CpG, p<5×10–8) |
| Obesity(-related) CpGs known in EWAS* | ||||||
| cg06500161 | 21:43 656 587 | ABCG1 | Body | BMI, TG, WC, T2D, Fasting Glucose | T2D, BMI, Blood TG, MetS | – |
| cg27243685 | 21:43 642 366 | ABCG1 | 5'UTR | BMI, TG, WC, Total lipids | BMI | – |
| cg01082498 | 11:68 608 225 | CPT1A | 5'UTR | TG, LDL cholesterol | LDL cholesterol, Blood TG | BMI |
| cg14465376 | 3:81 766 440 | GBE1 | Body | TG, Cholesterol, HC | – | BMI, WC |
| cg13001289 | 17:1 415 550 | INPP5K | Body | Total cholesterol | Obesity | – |
| cg02335306 | 11:2 559 880 | KCNQ1 | Body | Free cholesterol to total lipids ratio in small VLDL | – | T2D |
| cg16744911 | 10:102 108 974 | SCD | Body | – | Birth weight | Fatty acid desaturase activity (fat tissue), Total cholesterol |
| cg11024682 | 17:17 730 094 | SREBF1 | Body | BMI, TG, CRP, WC, T2D | BMI, WC, Blood TG, T2D | BMI, T2D |
| Novel CpGs but known gene in GWAS† | ||||||
| cg20655350 | 2:219 124 263 | GPBAR1 | TSS1500 | – | – | WHR |
| cg04321608 | 22:50 689 962 | HDAC10 | TSS200 | – | – | BMI |
| cg17827949 | 2:207 974 984 | MIR2355;KLF7 | TSS200;Body | – | – | BMI |
| cg18706511 | 6:46 350 774 | RCAN2 | Body | – | – | BMI |
| cg11884741 | 11:134 449 995 | – | – | – | – | BMI |
| cg23386509 | 16:80 843 728 | – | – | – | – | WHR |
| cg23891633 | 12:11 695 118 | – | – | – | – | Subcutaneous adipose tissue |
| cg27413950 | 14:60 789 354 | – | – | – | – | Hip circumference adjusted for BMI |
| Novel CpGs but known gene in GWAS† related to complex trait | ||||||
| cg01269795 | 6:26 440 101 | BTN3A3 | TSS1500 | – | – | TG |
| cg13424229 | 3:148 581 837 | CPA3 | TSS1500 | – | – | Birth weight |
| cg00857282 | 6:16 130 727 | MYLIP | Body | – | – | Cholesterol, LDL cholesterol, Total cholesterol |
| cg13980454 | 14:36 985 905 | NKX2-1 | 3'UTR | – | – | T2D |
| cg14546197 | 17:38 137 043 | PSMD3 | TSS200 | – | – | Subcutaneous adipose tissue |
| cg03904213 | 21:40 457 407 | – | – | – | – | TG |
| Novel CpGs in both GWAS† and EWAS* | ||||||
| cg16740586 | 21:43 655 919 | ABCG1 | Body | – | – | – |
| cg14234394 | 1:212 787 866 | ATF3 | 5'UTR;TSS1500 | – | – | – |
| cg23762306 | 16:3 817 973 | CREBBP | Body | – | – | – |
| cg05779336 | 16:23 682 007 | DCTN5 | Body | – | – | – |
| cg01579884 | 7:37 004 390 | ELMO1 | 5'UTR | – | – | – |
| cg21739530 | 4:174 186 068 | GALNT7 | Body | – | – | – |
| cg03134285 | 1:67 836 673 | IL12RB2 | Body | – | – | – |
| cg05935283 | 3:190 231 667 | IL1RAP | TSS200 | – | – | – |
| cg17909892 | 10:135 009 388 | KNDC1 | Body | – | – | – |
| cg13279894 | 4:151 600 922 | LRBA | Body | – | – | – |
| cg22796849 | 19:17 530 340 | MVB12A | TSS1500 | – | – | – |
| cg19554457 | 12:93 774 772 | NUDT4 | Body | – | – | – |
| cg23610075 | 12:14 722 220 | PLBD1 | TSS1500 | – | – | – |
| cg13689699 | 9:137 225 311 | RXRA | Body | – | – | – |
| cg03001463 | 2:200 323 278 | SATB2 | 5'UTR | – | – | – |
| cg19842683 | 10:102 795 320 | SFXN3 | Body | – | – | – |
| cg15833511 | 20:35 256 798 | SLA2 | Body | – | – | – |
| cg26257411 | 6:33 397 122 | SYNGAP1 | Body | – | – | – |
| cg26547928 | 12:33 589 385 | SYT10 | Body | – | – | – |
| cg27465654 | 2:71 221 575 | TEX261 | Body | – | – | – |
| cg10851763 | 21:43 545 792 | UMODL1 | Body | – | – | – |
| cg23487560 | 2:10 832 689 | – | – | – | – | – |
| cg02006035 | 9:118 859 925 | – | – | – | – | – |
| cg15730234 | 1:101 759 418 | – | – | – | – | – |
| cg21098652 | 11:121 129 115 | – | – | – | – | – |
| cg24271501 | 6:47 042 976 | – | – | – | – | – |
IDs of CpG sites and genes in bold indicate that differential methylation was correlated with differences of gene expression, and each obesity (-related) CpGs were differentially methylated in both blood and fat tissue.
*EWAS: EWAS catalog (http://www.ewascatalog.org/) and EWAS atlas (https://bigd.big.ac.cn/ewas).
†GWAS: GWAS catalog (https://www.ebi.ac.uk/gwas/).
BMI, body mass index; CRP, C-reactive Protein; EWAS, epigenome-wide association study; GWAS, genome-wide association studies; HC, Hepatic Cholesterol; LDL, Low-density lipoprotein; T2D, type 2 diabetes; TG, Triglyceride; WC, Waist Circumference; WHR, waist-hip ratio.