Figure 5.

GC–MS analysis of the products formed in vitro by the enzymatic activities of tomato terpene synthase (TPS) proteins using geranyl diphosphate (GPP) (a) and dimethylallyl diphosphate (DMAPP) (b) as substrates. Reaction products were collected and analysed as described in the ‘Materials and Methods’ section, commercial isoprene was used as a standard. Reaction products were identified by comparison of their mass spectra and retention indices with authentic standards and NIST libraries: 25, β‐myrcene; 26, β‐cis‐ocimene; 27, β‐trans‐ocimene; 28, linalool; 29, isoprene. Mass spectra and retention indices of the terpene products can be found in Supporting Information Fig. S3; Table S2, respectively.