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DAPI staining of the decellularized posterior poles and non-decellularized controls. DAPI staining was performed for the control and decellularization protocols with high triton for 1 hour, low triton for 1 hour, low triton for 48 hours, low triton for 1 week, no trypsin, and high trypsin. The resulting images confirmed the DNA quantification results in that the long duration triton treatments better cleared the tissues of cellular material. Scale bar = 100 µm.
