Figure 7.

Pressurization of the posterior poles before and after decellularization and treatment with hydrogel or super-glue. Pressurizing the posterior poles to 15 mm Hg showed a significantly lower proportion (%) of the tissues were able to hold pressure after decellularization, but infiltrating the decellularized tissues with a PEG-based hydrogel enabled them to retain this ability (A). Measuring the displacement of the tissues after increasing the pressure on the tissues from 5 to 15 mm Hg showed that treating the decellularized tissues with either PEG 6000 or PEG 10,000 restored the biomechanical properties of tissue to that prior to decellularization (B). Dissect samples refers to the matched, non-decellularized controls, and displacement was normalized to the paired dissect displacement. *P ≤ 0.05.