Table.
Decellularization Protocols Evaluated in This Study
Triton X-100 | Trypsin/EDTA | |||
---|---|---|---|---|
Group | Content | Duration | Content | Duration |
High Triton, 1 h | 3.0% | 1 h | 0.02% Trypsin/0.05% EDTA | 1 h |
Low Triton, 1 h | 0.1% | 1 h | 0.02% Trypsin/0.05% EDTA | 1 h |
Low Triton, 48 h | 0.1% | 48 h | 0.02% Trypsin/0.05% EDTA | 1 h |
Low Triton, 1 wk | 0.1% | 1 wk | 0.02% Trypsin/0.05% EDTA | 1 h |
No Trypsin | 3.0% | 1 h | 0% Trypsin/0% EDTA | 1 h |
High Trypsin | 3.0% | 1 h | 0.1% Trypsin/0.25% EDTA | 1 h |