Table 2.
Decalcifying solution scores as the measurement of decalcification time and morphological fungi preservation.
| Decalcifying solutions | Decal/time RT/MW Hours/minutes |
M. mycetomatis fungi morphological evaluation | Total score | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| FSHE, RT/MW | FSG, RT/MW | FSGr, RT/MW | ||||||||||||
| P | F | G | E | P | F | G | E | P | F | G | E | |||
| 10% EDTA | 120 h/29 h: 4 min | 3/1 | 5/2 | 10/4 | 32/43 | 1/5 | 1/7 | 15/6 | 33/32 | 1/0 | 1/0 | 5/9 | 43/41 | 50/50 |
| 5% nitric acid | 7 h: 30min/3:45 min | 2/3 | 2/3 | 37/2 | 9/42 | 5/3 | 4/4 | 8/4 | 33/39 | 1/3 | 2/4 | 15/9 | 32/34 | 50/50 |
| 5% HCl | 8 h/5 h: 30 min | 2/2 | 5/4 | 42/11 | 1/33 | 5/1 | 5/1 | 18/17 | 22/31 | 5/2 | 4/2 | 18/11 | 23/35 | 50/50 |
| Chi-square test | P value: 0.023 | P value: 0.001 | P value: 0.03 | |||||||||||
Note. Decal: decalcification. FSHE: fungi staining with haematoxylin and eosin. FSG: fungi staining with Gridley stain. FSGr: fungi staining with Grocott hexamine-silver stain. RT: room temperature. MW: microwave oven. P: poor. F: fair. G: good. E: excellent.