. Author manuscript; available in PMC: 2021 Jul 20.

Published in final edited form as: Chem Res Toxicol. 2020 Jun 18;33(7):1689–1697. doi: 10.1021/acs.chemrestox.9b00514

Table 1.

Summary of Inhibition of Microsomal Recombinant CYP2A6 by Selected E-Liquids at 60 Minutes.^a

Lowest Concentration Tested	Highest Concentration Tested	E-Liquid Name	IC₅₀ of 3-cyano coumarin 7-hydroxylation	IC₅₀ 95% confidence interval (lower limit-upper limit)
0.000031%	0.125%	Apple Watermelon	>0.125%	--
0.000031%	0.125%	Strawberry Poptart	0.0058%	0.0048–0.0069%
0.000031%	0.125%	Flamethrower	0.00038%	0.00025–0.00052%

For screening of e-liquids impact on microsomal recombinant CYP2A6 activity, each concentration was screened on three separate days in duplicate (n=3).