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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Curr Protoc Microbiol. 2020 Sep;58(1):e107. doi: 10.1002/cpmc.107

Figure 1. Transfection of HAE-ALI cultures using lentiviral vectors.

Figure 1.

Proliferating human airway epithelial cells (as monolayer) cultured in SAGM-H medium are transferred onto Transwell® inserts. Two days later, the cells are transduced with lentiviruses and cultured in SAGM-H medium for 2 days. On day 4, the medium in the inserts (apical chamber) is removed, and only the basolateral chamber is fed with PneumaCut ALI medium. The cells are further cultured at an ALI for 3 to 4 weeks for fully differentiation before ready for use in virus infection.