Table 1.
Precision, Accuracy, bias and recovery data for 17OHP in bloodspots by LC-MSMS. * Target value assigned by the CDC. ** LCMSMS method mean (n = 79) from laboratories submitting analytical data to the CDC.
| Precision Data (n = 24) | |||
|---|---|---|---|
| Control 1 | Control 2 | Control 3 | |
| Within-Batch (n = 20) | |||
| Mean (nmol/L) | 14.6 | 74.9 | 155.7 |
| CV% | 13.7 | 7.0 | 6.2 |
| Between-Batch (n = 22) | |||
| Mean (nmol/L) | 15.0 | 74.9 | 155.1 |
| CV% | 11.9 | 6.1 | 9.3 |
| Accuracy | |||
| Level 1 | Level 2 | Level 3 | |
| CDC Target Value (nmol/L) * | 15 | 66 | 134 |
| Inter-laboratory Mean (nmol/L) ** | 17 | 82 | 162 |
| Mean (nmol/L) (n = 10) | 19 | 77 | 164 |
| Recovery | |||
| Level 1 | Level 2 | Level 3 | |
| Baseline Level | ND | ND | ND |
| Enrichment (nmol/L) | 15.2 | 76.0 | 152 |
| Mean | 15.0 | 74.9 | 155 |
| Recovery (%) | 101% | 98.6% | 102% |