Fig 4. L. sigmodontis-specific Ig response is not altered by the absence of mast cells.

Mast cell deficient Cpa3^cre and their cell-competent littermates Cpa3^wt mice were infected with L. sigmodontis for 40 and 75 days. A) LsAg-specific IgE was analyzed by ELISA in the thoracic cavity lavage (A) and the serum (B) of infected mice. LsAg-specific IgG2a (C), IgG2b (D), and IgG1 (E) was measured in the serum of infected Cpa3^cre and Cpa3^wt mice. Data show the mean and are combined from two independent experiments. Each dot represents the Ig response from a single mouse (A: day 75: n = 7 Cpa3^wt and n = 10 Cpa3^cre; B day 40: n = 12 Cpa3^wt and n = 9 Cpa3^cre; day 75: n = 9 Cpa3^wt and n = 12 Cpa3^cre; C-E: day 40: n = 10 Cpa3^wt and n = 9 Cpa3^cre; day 75: n = 9 Cpa3^wt and n = 12 Cpa3^cre mice). The less abundant isotypes IgE and IgG2 are expressed as a.u., IgG1 data is depicted as titer. Data were analyzed by unpaired student t test or Mann-Whitney U test.