Black, grey, and white circles on nodes represent bootstrap values of 100%, greater than or equal to 80% to less than 100%, and greater than or equal to 50% and less than 80%, respectively. Blue circles represent Platanus assemblies, red circles represent the reference assembly, and yellow circles represent SOAPdenovo2 assemblies. The numbers next to each circle depicts the step-size or k-mer size for each assembly. Roman numerals identify isolog clusters. Scale bar represents substitutions per site. Asterisks indicate two matches that represent the single case of fragmented protein prediction from the SOAPdenovo2 k-mer 63 assembly in this tree.