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. 2020 Aug 12;15(8):e0237353. doi: 10.1371/journal.pone.0237353

Table 2. Mean percent escape response and percent mortality of An. minimus (DDC) exposed to TFT in ER assays.

% Concentration ER assay Percent escape response Percent knockdown and mortality in treatments***
Control Treatment % KD 30-min exposure % Mortality 24 h
N %Esc.* N %Esc.**,(§) Esc. NEsc. Esc. NEsc.
0.00423 (LC50) Noncontact 60 15.00a 64 60.07a 11.11a 26.32a 2.22a 0.0a
Contact 61 6.56a 60 85.73a (16.35) 1.92b 37.50a 0.0a 12.50a
0.00760 (LC75) Noncontact 60 11.67a 54 66.46a 18.42a 62.50a 0.0a 12.50a
Contact 59 10.17a 58 80.81a (12.38) 10.42a 80.00a 4.17a 60.00b
0.03191 (LC99) Noncontact 60 5.00a 58 54.63a 63.64a 80.00a 0.0a 12.00a
Contact 60 8.33a 62 52.49a (0) 65.71a 100.0a 2.86a 55.56b
0.06382 (DC) Noncontact 60 15.00a 60 58.82a 71.79a 71.43a 2.56a 14.29a
Contact 60 11.67a 60 43.39a (0) 83.33a 96.67b 26.67b 50.00b

ER, excito-repellency; Esc, escaped mosquitoes; NEsc, non-escaped mosquitoes; KD, knockdown; LC, lethal concentration; DC, discrinimating concentration.

*Percent escape in control group showed no significantly difference (P > 0.05) between noncontact and contact tests at the same concentration.

**Percent escape adjusted with paired controls using Abbott’s formula. Different letter indicates significant differences (P < 0.05) between noncontact and contact tests in treatment group at the same concentration.

***Different letter indicates significant differences (P < 0.05) between control, noncontact and contact tests at the same concentration and same response group (Esc. or NEsc.).

§Percent contact escape adjusted with paired noncontact escape response using modified Henderson-Tilton’s formula.

†Due to TFT vaporization during drying period, the actual percent concentration at the time of testing is less than initial paper treatment percent indicated.

30-min KD and 24-hr mortality not observed in controls.