Table 2. Mean percent escape response and percent mortality of An. minimus (DDC) exposed to TFT in ER assays.
% Concentration† | ER assay | Percent escape response | Percent knockdown and mortality in treatments*** | ||||||
---|---|---|---|---|---|---|---|---|---|
Control | Treatment | % KD 30-min exposure | % Mortality 24 h | ||||||
N | %Esc.* | N | %Esc.**,(§) | Esc. | NEsc. | Esc. | NEsc. | ||
0.00423 (LC50) | Noncontact | 60 | 15.00a | 64 | 60.07a | 11.11a | 26.32a | 2.22a | 0.0a |
Contact | 61 | 6.56a | 60 | 85.73a (16.35) | 1.92b | 37.50a | 0.0a | 12.50a | |
0.00760 (LC75) | Noncontact | 60 | 11.67a | 54 | 66.46a | 18.42a | 62.50a | 0.0a | 12.50a |
Contact | 59 | 10.17a | 58 | 80.81a (12.38) | 10.42a | 80.00a | 4.17a | 60.00b | |
0.03191 (LC99) | Noncontact | 60 | 5.00a | 58 | 54.63a | 63.64a | 80.00a | 0.0a | 12.00a |
Contact | 60 | 8.33a | 62 | 52.49a (0) | 65.71a | 100.0a | 2.86a | 55.56b | |
0.06382 (DC) | Noncontact | 60 | 15.00a | 60 | 58.82a | 71.79a | 71.43a | 2.56a | 14.29a |
Contact | 60 | 11.67a | 60 | 43.39a (0) | 83.33a | 96.67b | 26.67b | 50.00b |
ER, excito-repellency; Esc, escaped mosquitoes; NEsc, non-escaped mosquitoes; KD, knockdown; LC, lethal concentration; DC, discrinimating concentration.
*Percent escape in control group showed no significantly difference (P > 0.05) between noncontact and contact tests at the same concentration.
**Percent escape adjusted with paired controls using Abbott’s formula. Different letter indicates significant differences (P < 0.05) between noncontact and contact tests in treatment group at the same concentration.
***Different letter indicates significant differences (P < 0.05) between control, noncontact and contact tests at the same concentration and same response group (Esc. or NEsc.).
§Percent contact escape adjusted with paired noncontact escape response using modified Henderson-Tilton’s formula.
†Due to TFT vaporization during drying period, the actual percent concentration at the time of testing is less than initial paper treatment percent indicated.
30-min KD and 24-hr mortality not observed in controls.