Effect of Wy on BA composition in gallbladders of Pparafl/fl and PparaΔHep mice. A. Heat map of individual BA levels. B. Hydrophobicity index of bile acids in gallbladders. C. Total concentration of different BA classes. D. Relative percentage of different BA classes to total BAs. E. Relative fraction of individual BAs in gallbladders of chow-fed Pparafl/fl mice. F. Relative fraction of individual BAs in gallbladders of Wy-treated Pparafl/fl mice. G. Relative fraction of individual BAs in gallbladders of chow-fed PparaΔHep mice. H. Relative fraction of individual BAs in gallbladders of Wy-treated PparaΔHep mice. I. 12α-OH/non-12α-OH BAs ratio. Σ-BAs, total BAs. T-BAs, taurine-conjugated BAs. U-BAs, unconjugated BAs. 1° BAs, primary BAs. 2° BAs, secondary BAs. 12α-OH, 12α-hydroxylated BAs. 6-OH, 6-hydroxylated BAs. Data are presented as mean ± SEM; n = 3–4/group. *P < 0.05 or **P < 0.01, by one-way ANOVA followed by Tukey’s post-hoc correction.