Effect of fasting on hepatic BA composition in Pparafl/fl and PparaΔHep mice. A. Heat map of individual BA levels. B. Hydrophobicity index of hepatic bile acids. C. Total concentration of different BA classes. D. Relative percentage of different BA classes to total BAs. E. Relative fraction of individual BAs in livers of fed Pparafl/fl mice. F. Relative fraction of individual BAs in livers of fasted Pparafl/fl mice. G. Relative fraction of individual BAs in livers of fed PparaΔHep mice. H. Relative fraction of individual BAs in livers of fasted PparaΔHep mice. I. 12α-OH/non-12α-OH BAs ratio. Σ-BAs, total BAs. T-BAs, taurine-conjugated BAs. U-BAs, unconjugated BAs. 1° BAs, primary BAs. 2° BAs, secondary BAs. 12α-OH, 12α-hydroxylated BAs. 6-OH, 6-hydroxylated BAs. Data are presented as mean ± SEM; n = 6/group. *P < 0.05 or **P < 0.01, by one-way ANOVA followed by Tukey’s post-hoc correction.