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. Author manuscript; available in PMC: 2020 Aug 12.
Published in final edited form as: Methods Mol Biol. 2017;1632:219–230. doi: 10.1007/978-1-4939-7138-1_14

Fig. 3.

Fig. 3

In situ hybridization of miR-34a with small nuclear RNA U6 (snoU6) as endogenous control. Formalin-fixed, paraffin-embedded slides were stained with hematoxylin and eosin (H&E) or in situ hybridization was performed by using a double digoxigenin-labeled miRCURY locked nucleic acid probe designed for control miRNA or miR-34a after a digestion step with proteinase K. The mean (± standard deviation) intensities obtained from ten areas from each slide were measured using the NIS Elements software (reproduced from ref. 28 with permission from Oncotarget)