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. 2020 Aug 12;10(4):e139. doi: 10.1002/ctm2.139

FIGURE 6.

FIGURE 6

Effect of EV co‐culture on PI3K/AKT/mTOR signaling in LoVo and HT29 cells. LoVo‐CSCs and HT29‐CSCs were first transfected with miR‐200c mimics (miR‐200c‐mim) or inhibitors (miR‐200c‐inh). LoVo cells were co‐cultured for 48 h with EVs isolated from nontransfected (Control) or transfected (A) LoVo‐CSCs or (B) HT29‐CSCs, with or without ATL‐1 administration at 200 μM. Similarly, HT29 cells were co‐cultured for 48 h with EVs isolated from nontransfected (Control) or transfected (C) LoVo‐CSCs or (D) HT29‐CSCs, with or without ATL‐1 administration at 200 μM. Western blot and quantification of the phosphorylation levels of PI3K, AKT, and mTOR relative to the total level of the corresponding protein were carried out. Overexpression of miR‐200c in EVs enhanced the activation of the PI3K/AKT/mTOR signaling pathway in LoVo and HT29 cells via horizontal transfer, whereas miR‐200c interference suppressed these traits. The data represent the mean ± SD of three independent technical replicates (ANOVA); * P < .05; P < .05 versus the same group with PBS only (+PBS)