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Effect of protein concentration on the electron transfer activity measurement of cyt bc1 complex. Cytochrome bc1 complex preparation was dissolved in 50 mM Tris-HCl buffer, pH 8.0, containing 0.66 M sucrose and 0.01% DM at indicated protein concentrations. Three- to five-microliter aliquots were used for activity determination at room temperature. Specific activity is expressed as μmoles cytochrome c reduced/nmol cytochrome b/min.
