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. Author manuscript; available in PMC: 2020 Aug 12.
Published in final edited form as: Methods Enzymol. 2009;456:459–473. doi: 10.1016/S0076-6879(08)04425-X

Figure 25.2.

Figure 25.2

Proton-pumping of cyt bc1 complexes embedded in PL vesicles. Measurement of pH change as an indicator for the proton-pumping activity of PL vesicles embedded with (A) mitochondria bc1 complex only, (B) with mitochondrial bc1 complex and the 2Fe-2S cluster lacking mutant complex, H131C-H152C, with ubiquinol as the substrate. Arrows indicate the points of addition of 5 nmol of ferricyanide (1), 3 μM CCCP (2), 5 nmol of ferricyanide (3), and 5 nmol HCl (4). Note: proton-pumping ratio (H+/e− ) = x/y.

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