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. 2020 Aug 7;13:7851–7864. doi: 10.2147/OTT.S253485

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© 2020 Li et al.

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P-ERK and p-AKT in tumor-derived exosomes regulate IL-6 secretion. (A) ELISA was used to detect the concentration of IL-6 in the cultured medium of HSCs and aHSCs (LoVo and HCT116 exosome-treated HSCs). (B) PCR and Western blotting for the IL-6 expression of HSCs and tumor-derived exosomes pre-treated HSCs. (C) IHC was performed to analyze α-SMA and IL-6 in the liver metastases of colorectal cancer patients. (D) Western blotting analysis of the expression level of p65 in the cytoplasm and nucleus of HSCs and aHSCs. (E) ELISA was used to detect the concentration of IL-6 in the medium of HSCs and aHSCs, with or without NF-κB inhibitors (BAY11-7082). (F) Western blotting analysis of the expression of p-ERK, ERK, p-AKT and AKT in HSCs and aHSCs. (G) Western blotting analysis of the expression of p-ERK, ERK, p-AKT and AKT in the exosomes of LoVo and HCT116. (H) ELISA was used to detect the concentration of IL-6 in the cultured medium of HSCs and aHSCs treated with MK226 and U0126. N=3; ns, no significant difference; *P<0.05; **P<0.001.