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. 2020 Aug 3;13:7555–7569. doi: 10.2147/OTT.S254489

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© 2020 Wang et al.

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SphK1 is targeted by miR-613 in HT29 and LOVO cells. (A) Putative miR-613 binding sequences in the 3ʹ-UTR of SphK1 and the reporter constructs showing the wild type (Wt) SphK1 3ʹ-UTR sequence and the mutant (Mut) SphK1 3ʹ-UTR sequence. (B and C) miR-613 mimics transfection suppressed the luciferase activity of the Wt but not the MUT SphK1 3ʹ-UTR reporter in HT29 and LOVO cells. (D) qRT-PCR analysis and (E) Western blot analysis of SphK1 mRNA and protein expression levels in HT29 and LOVO cells after miR-NC or miR-613 mimics transfection. (F) qRT-PCR analysis and (G) Western blot analysis of SphK1 mRNA and protein expression levels in HT29 and LOVO cells after si-NC or si-LINC00460 transfection. **p < 0.01.