Table 2.
Real-Time PCR Primers Used for mRNA Expression Analysis
| Target Genes | Primer (5ʹ-3ʹ) | |
|---|---|---|
| LINC00460 | Forward | 5ʹ-GTGGATGAGAACGAAGGTTACG-3’ |
| Reverse | 5ʹ-CTTTCCCACGCTCAGTCTTT-3’ | |
| miR-613 | Forward | 5ʹ-CGCAGCACACCTGCTTTTTG-3’ |
| Reverse | 5ʹ-AGAGATTCGGGTCGATGCTC-3’ | |
| Sphk1 | Forward | 5ʹ-CCTTCCTCCTTCCCTAGGG-3’ |
| Reverse | 5ʹ-TAGAAGGCCTTACATAGGCAGC-3’ | |
| U6 | Forward | 5ʹ-TCCGATCGTGAAGCGTTC-3’ |
| Reverse | 5ʹ-GTGCAGGGTCCGAGGT-3’ | |
| GAPDH | Forward | 5ʹ-AAGGTGAAGGTCGGAGTCAA-3’ |
| Reverse | 5ʹ-AATGAAGGGGTCATTGATGG-3’ | |
Notes: U6 as the internal reference of LINC00460 and miR-613; GAPDH as the internal reference of Sphk1.