Table 1.
The potential CD8+ T-cell epitopes along with their interacting MHC class I alleles and total processing score, epitopes conservancy hits and pMHC-I immunogenicity score.
| Epitopes | NetCTL Combined score |
Epitope_ Conservancy_Hit (MAX. Identity %) |
MCH-I interaction with an affinity of IC50 and the total score (proteasome score, TAP score, MHC-I score, processing score) |
pMHC-I immunogenicity score |
|---|---|---|---|---|
| WTAGAAAYY | 3.1128 | 100 | HLA-A*29:02 (1.51), HLA-A*26:01 (1.43), HLA-A*68:01 (1.12), HLA-C*12:03 (0.99), HLA-B*15:25 (0.97), HLA-B*35:01 (0.96), HLA-C*03:02 (0.91), HLA-A*30:02 (0.90), HLA-A*01:01 (0.89), HLA-B*15:01 (0.78), HLA-B*15:02 (0.68), HLA-C*16:01 (0.62), HLA-A*25:01 (0.56), HLA-C*02:09 (0.53), HLA-C*02:02 (0.53), HLA-C*12:02 (0.52), HLA-C*14:02 (0.24) | 0.15259 |
| CNDPFLGVY | 1.3355 | 100 | HLA-A*01:01 (0.36) | 0.15232 |
| GAAAYYVGY | 1.2194 | 100 | HLA-B*15:25 (1.03), HLA-A*29:02 (0.81), HLA-B*15:01 (0.69), HLA-A*30:02 (0.56), HLA-B*15:02 (0.39) | 0.09963 |
| ITDAVDCAL | 1.1680 | 100 | HLA-C*05:01 (0.62), HLA-C*08:02 (0.13), HLA-C*08:01 (0.08), HLA-C*03:04 (−0.41), HLA-C*03:03 (−0.41), HLA-C*16:01 (−0.43) | 0.08501 |
| STQDLFLPF | 1.0468 | 100 | HLA-B*15:25 (0.77), HLA-B*15:01 (0.60), HLA-B*15:02 (0.50), HLA-A*32:01 (0.48), HLA-C*16:01 (0.42), HLA-C*03:02 (0.35), HLA-B*35:01 (0.02), HLA-C*12:03 (−0.03), HLA-A*29:02 (−0.11) | 0.06828 |
| TSNQVAVLY | 3.0758 | 100 | HLA-A*01:01 (0.76), HLA-A*29:02 (0.68), HLA-A*30:02 (0.54), HLA-B*58:01 (0.33) | −0.01327 |
| KTSVDCTMY | 2.3795 | 100 | HLA-A*30:02 (1.04), HLA-B*58:01 (0.47) | −0.11115 |
| MTSCCSCLK | 1.0963 | 100 | HLA-A*68:01 (0.26), HLA-A*11:01 (−0.07), HLA-A*03:01 (−0.74), HLA-A*30:01 (−0.92), HLA-A*31:01 (−0.93), HLA-A*33:03 (−1.19) | −0.36816 |
| STECSNLLL | 2.3492 | 100 | HLA-C*05:01 (−0.27) | −0.20478 |
| GAEHVNNSY | 1.9960 | 100 | – | −0.00296 |
Notes: MHC-I alleles that have an interacting affinity lower than 200 nm are represented, and total processing scores are shown as enclosed numbers.