Table 4.
Epitope | Species | Catalog No. | Company | Use | Dilution | Source |
---|---|---|---|---|---|---|
Primary antibodies | ||||||
α-tubulin | human | # T-9026 | Sigma-Aldrich | WB | 1:1000 | mouse |
β-actin | human | # A2066 | Sigma-Aldrich | WB | 1:1000 | rabbit |
p53 | human | # sc-126 | Santa Cruz | WB | 1:1000 | mouse |
E-cadherin | human | # 334000 | Invitrogen | WB | 1:1000 | mouse |
CSF1R | human | # HPA012323 | Sigma-Aldrich | WB | 1:1000 | rabbit |
Vimentin | human | # 2707-1 | Epitomics | WB | 1:1000 | rabbit |
SNAIL | human | # 3879S | Cell Signaling | WB | 1:500 | rabbit |
ZEB1 | human | # sc-25388 | Santa Cruz | WB | 1:1000 | rabbit |
STAT3pS727 | human | # 9134 | Cell Signaling | WB | 1:1000 | rabbit |
STAT3 | human | # sc-482 | Santa Cruz | WB | 1:1000 | rabbit |
VSV | human | # V4888 | Sigma-Aldrich | WB; ChIP | 1:1000 | rabbit |
CSF1R | human | # sc-692 | Santa Cruz | WB | 1:500 | rabbit |
CSF1R | human | # ab183316 | Abcam | IHC | 1:100 | rabbit |
Secondary antibodies | |||||
---|---|---|---|---|---|
Name | Ordering No. | Company | Use | Dilution | Source |
anti-mouse HRP | # W4021 | Promega | WB | 1:10.000 | goat |
anti-rabbit HRP | # A0545 | Sigma | WB | 1:10.000 | goat |
ChIP, chromatin immunoprecipitation; IHC, immunohistochemistry; WB, Western blot analysis.