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Oligonucleotides used for MSP and BSP
| Forward (5′-3′) | Reverse (5′-3′) | |
|---|---|---|
| MSP_M | GGTTTTGGGTAGGCGCGTTTC | TCCTCATCCCCTTCACCGCCG |
| MSP_U | (Inosine)(Inosine)GGTTTTGGGTAGGTGTGTTTT | AATCCTCATCCCCTTCACCACCA |
| BSP_1 | TAGAGATAATAGGTTTTGATTCGGGATAGA | CAAAACTCCCACAAAATCTCCAAA TACCCCC |
| BSP_2 | TAGAGATAATAGGTTTTGATTTGGGATAGA | CAAAACTCCCGCAAAATCTCCAAA TACCCCC |
BSP, bisulfite-sequencing polymerase chain reaction; MSP, methylation-specific polymerase chain reaction.
