Table 2.

Sample setup, including various standard proteoglycans and proteins, and mouse brain tissue lysate used for testing the protocol for each step i-iv (see Fig 2). HSBK; heparan sulfate from bovine kidney, HSPIM; Heparan sulfate from porcine intestinal mucosa, AGP; α-Acid Glycoprotein.

Batch 1	Batch 2	Targeted biomolecules	Steps
Lysate (100 µgx1)	Lysate (100 µgx1)	HA, CS, HS, peptides	i, ii, iii, iv
Hyaluronan (100 µgx1)	Hyaluronan (100 µgx1)	HA	i
Syndecan-1 (20 µgx1)	Syndecan-2 (20 µgx1)	CS, HS (only for batch1),peptides	ii, iii, iv
Aggrecan (20 µgx1)	Aggrecan (20 µgx1)	CS, peptides	ii, iv
Neurocan (20Ugx1)	-	CS, peptides	ii, iv
HSBK (100 µgx1)	HSBK (100 µgx1)	HS	iii
HSPIM (100 µgx1)	HSPIM (100 µgx1)	HS	iii
AGP (30 µgx1)	AGP (30 µgx1)	peptides	iv