Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 12;10:13623. doi: 10.1038/s41598-020-70675-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Influence of l-arginine biosynthesis on expression of rpoS and cfcR. (a) KT2440 (circles), and the ΔargG (triangles) and ΔargH (squares) strains harbouring pMAMV21 (rpoS’–‘lacZ) were grown in LB and β-galactosidase activity was measured at the indicated times. Data correspond to averages and standard errors of two biological replicas with three technical repetitions each. Statistically significant differences between the wild type and mutants were detected from 10 h onwards (Student´s t test: p < 0.05). (b) Influence of increasing concentrations of l-arginine on expression of rpoS’–‘lacZ in KT2440 and the ΔargG and ΔargH strains harbouring pMAMV21. Cultures were grown for 24 h in LB or LB with increasing concentrations of l-arginine (shown as increasing intensity colour bars) and β-galactosidase activity was analysed. Graph corresponds to averages and standard deviations of two biological replicas with three technical repetitions each. Statistically significant differences with respect to each control without amino acid supplementation are indicated by asterisks (Student´s t test: p ≤ 0.01). (c) KT2440 (circles), and the ΔargG (triangles) and ΔargH (squares) strains harbouring pMIR200 (cfcR::lacZ) were grown in LB and β-galactosidase activity was measured at the indicated times. Data correspond to averages and standard errors of two biological replicas with three technical repetitions each.