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. 2020 Aug 12;10:13623. doi: 10.1038/s41598-020-70675-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Role of substrate binding proteins in modulation of c-di-GMP cell content by environmental l-arginine. P. putida KT2440 (blue lines), ΔargT (crimson lines), ΔartJ (green lines), ΔoccT (orange lines), and ΔargTΔartJ (purple lines) strains harbouring pCdrA::gfp^C were grown in M9 minimal medium with glucose (a) and 5 mM (b) or 15 mM (c) l-arginine. Data correspond to fluorescence values corrected by culture growth (OD₆₆₀). Measurements were done every 30 min on a Varioskan Lux fluorimeter. Averages and standard deviations are plotted.