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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Mol Immunol. 2020 Jul 10;125:115–122. doi: 10.1016/j.molimm.2020.06.029

Figure 2. c-Rel knockout single-clonal Jurkat cells show reduced cell growth.

Figure 2.

A. Western blots of control and c-Rel knockout (KO) Jurkat clones. Each lane represents a single clone. B. control and c-Rel KO Jurkat cells were stimulated with or without PMA and ionomycin for 4 hours, followed by RT-PCR assay for human IL-2 (n=3 independent cultures of pooled clones). C. proliferation of control and c-Rel knockout Jurkat T cells in the culture (n=3 clones). D. Western blots of c-Rel KO Jurkat cell clones transduced with lentiviral control vector, or c-Rel-expressing vector. Each lane represents a single clone. E. cells were stimulated with PMA/ionomycin for 4 hours, followed by RT-PCR quantification of IL-2 (n=3 independent cultures of the indicated clones). F. proliferation of the indicated cells (n=3 clones). For all panels, data are expressed as mean ± SEM, and are representative of at least 3 independent experiments. *, p<0.05, **, p<0.01, ***, p<0.001.