A. Western blots of control and c-Rel knockout (KO) Jurkat clones. Each lane represents a single clone. B. control and c-Rel KO Jurkat cells were stimulated with or without PMA and ionomycin for 4 hours, followed by RT-PCR assay for human IL-2 (n=3 independent cultures of pooled clones). C. proliferation of control and c-Rel knockout Jurkat T cells in the culture (n=3 clones). D. Western blots of c-Rel KO Jurkat cell clones transduced with lentiviral control vector, or c-Rel-expressing vector. Each lane represents a single clone. E. cells were stimulated with PMA/ionomycin for 4 hours, followed by RT-PCR quantification of IL-2 (n=3 independent cultures of the indicated clones). F. proliferation of the indicated cells (n=3 clones). For all panels, data are expressed as mean ± SEM, and are representative of at least 3 independent experiments. *, p<0.05, **, p<0.01, ***, p<0.001.