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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Mol Immunol. 2020 Jul 10;125:115–122. doi: 10.1016/j.molimm.2020.06.029

Figure 4. c-Rel drives c-Myc gene expression in human Jurkat T cells.

Figure 4.

A. RT-PCR quantification of c-Myc and its target genes (SLC2A1, PPARGC1 A, and TFAM; n=3 independent cultures of pooled clones). B. Western blotting for c-Rel and c-Myc (Left panels) with quantification is shown on the right (n=3 clones). C. Western blots of c-Rel KO Jurkat cell clones transduced with GFP-expressing lentiviral control vector, or c-Rel- and GFP-expressing vector (Left panels), with quantification shown on the right (n=3 clones). D. c-Rel KO cells transduced with GFP and c-Rel KO cells transduced with c-Rel and GFP were stimulated with PMA/ionomycin for 4 hours, followed by RT-PCR quantification of c-Myc mRNA (n=3 independent cultures of the indicated clones). E. control and c-Rel KO Jurkat cells were transfected with a luciferase reporter plasmid containing either no promoter or a 2kb human c-Myc promoter, and stimulated overnight with PMA and ionomycin followed by luciferase assay (n=3 independent cultures of pooled clones). For all panels, data are expressed as mean ± SEM, and are representative of at least 3 independent experiments. *, p<0.05, **, p<0.01, ***, p<0.001.