Leptin-induced STAT3 phosphorylation in the hypothalamus of control and tanycyte-ablated mice. (a) Representative images of pSTAT3 immunohistochemistry 15 min after i.p. leptin injection. (b) Quantification of the pSTAT3-positive neurons in the images shown (a), n = 3–4. No significance found, two-way ANOVA followed by Sidak’s multiple comparison. ArcN, arcuate nucleus; DMH, dorsomedial nucleus; ME, median eminence; VMH, ventromedial nucleus; 3 V, third ventricle. Scale bar: 100 μm (a) [Color figure can be viewed at wileyonlinelibrary.com]