Figure 5.

Indirect calorimetry analysis of tanycyte-ablated mice at different housing temperatures. (a) Experimental schedule for indirect calorimetry after dietary tamoxifen administration. (b) Body weights of animals subjected to indirect calorimetry. (c, d) Fat and lean mass measured by EchoMRI at 4 weeks following initiation of tamoxifen diet or after 2 weeks of temperature change. (e) Food intake during metabolic profiling. (f) Representative images of fat pads of wildtype (WT) and tanycyte-ablated mice (Tny-DTA) at thermoneutrality. (g) Quantification of adipocyte size in gWAT and iWAT (f, left and middle panel, respectively), n = 7 per group. (h) Quantification of the lipid droplet size in BAT (f, right panel), n = 7 per group. (i) Respiratory exchange ratio (RER, VCO₂/VO₂) is measured at different temperature conditions. *, significant difference (p < .05, Student’s t test) between groups analyzed for more than 30 min, n = 8–10 per group. (j) Energy expenditure (EE, kcal/kg/hr), measured along with RER(I) at different temperature conditions, n = 8–10 per group. BAT, brown adipose tissue; gWAT, gonadal white adipose tissue; iWAT, inguinal white adipose tissue. *p < .04, **p < .001, two-way ANOVA with Sidak’s multiple comparison test; n, as indicated for (b–e)