KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Monoclonal mouse anti-Ser/Thr-Pro MPM-2 | Upstate Biotechnology, Lake Placid, NY | Davis et al., 1983 |
| Affinity-purified rabbit anti-Mxc | Pocono Rabbit Farm & Laboratory, Inc. | White et al., 2011 |
| Affinity-purified guinea pig anti-FLASH | Pocono Rabbit Farm & Laboratory, Inc. | White et al., 2011 |
| Halocarbon Oil 27 | Sigma-Aldrich | CAS Number 9002-83-9 (H8773) |
| Halocarbon Oil 700 | Sigma-Aldrich | CAS Number 9002-83-9 (H8898) |
| Chemicals, Peptides, and Recombinant Proteins | ||
| SNS-032 | Selleck Chemicals | Catalog No S1145 |
| Alpha-amanitin | Sigma-Aldrich | CAS Number 23109-05-9 |
| Roscovitine | Sigma-Aldrich | CAS Number 186692-46-6 |
| Aqua-Poly/Mount | Polysciences, Inc. | Cat #: 18606 |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster; w; DHB-mCherry | This paper | N/A |
| D. melanogaster; w; ubi-gfp::mxc | R. J. Duronio (White et al., 2011) | N/A |
| D. melanogaster; w; ubi-gfp::mxcLisH-AAA | R. J. Duronio (Terzo et al., 2015) | N/A |
| D. melanogaster; w; ΔHisC, twi-GAL4/CyO | R. J. Duronio (McKay et al., 2015) | N/A |
| D. melanogaster; w; ΔHisC/ΔHisC; 12xWT/12xWT | R. J. Duronio (McKay et al., 2015) | N/A |
| D. melanogaster; w; grp209 lok30/grpz5170 lok30 | J. Sekelsky (LaRocque et al., 2007) | University of North Carolina at Chapel Hill |
| D. melanogaster; w; SLBP[12]/TM3, Sb[1] Ser[1] Tw | R. J. Duronio (Sullivan et al., 2001) | N/A |
| D. melanogaster; w[1118] | A. Bejsovec | Duke University |
| D. melanogaster; w[*]; CycA[C8LR1]/TM3, Sb[1] P{w[+mC]=35UZ}2 | Bloomington | BDSC: 6627 FlyBase: FBst0006627 |
| D. melanogaster; w[*]; CycB[2]/CyO, P{ry[+t7.2]=ftz/lacB}E3 | Bloomington | BDSC: 6630 FlyBase: FBst0006630 |
| D. melanogaster; w[*]; Cdk2[2]/TM3, Sb[1] P{w[+mC]=35UZ}2 | Bloomington | BDSC: 6632 FlyBase: FBst0006632 |
| D. melanogaster; w[*]; Cdk2[3]/TM3, Sb[1] P{w[+mC]=35UZ}2 | Bloomington | BDSC: 6636 FlyBase: FBst0006636 |
| D. melanogaster; w[*]; CycE[AR95] cn[1] pr[1] bw[1] wx[wxt]/CyO, P{ry[+t7.2]=ftz/lacB}E3 | Bloomington | BDSC: 6637 FlyBase: FBst0006637 |
| D. melanogaster; y[1] w[67c23]; P{y[+mDint2] w[BR.E.BR]=SUPor-P}CycE[KG00239]/SM6a | Bloomington | BDSC: 13071 FlyBase: FBst0013071 |
| D. melanogaster; w; EGFP-Slp1-BAC | This manuscript | N/A |
| D. melanogaster; w mxcG48/FM7C, twi-gfp | R. J. Duronio (White et al., 2011) | N/A |
| D. melanogaster; w gfp-mxc | R. J. Duronio (This manuscript) | N/A |
| Oligonucleotides | ||
| See Supplementary Table S1 for information on all oligonucleotides used in this study | N/A | N/A |
| Software and Algorithms | ||
| MATLAB R2018a | Mathworks | N/A |
| Adobe Illustrator CC 2018 | Adobe | N/A |
| JMP | https://www.jmp.com | N/A |