Fig. 3.

Comparative RNA-binding proteome profiling and functional categorization of MS results. RNA-binding proteome was isolated and identified from the human brain frontal cortical region of 20 cases (spAD, rpAD, sCJD-MM1, sCJD-VV2 as well as controls). a Venn diagram representing unique and shared RBP candidates in all groups. b Functional classification of MS results: RBP candidates identified in each group were analysed for associated Gene ontology functional-terms in two domains “biological process and molecular function’’. The protein counts associated with GO-terms from each group were uploaded to Perseus software, to prepare heatmap showing relative enrichment of different functional categories across all the groups. The variation in each term across the groups was calculated by Z-score. The columns of heatmap are representing disease groups (vertical-axis) and rows are displaying functional terms (x-axis), with red indicating an enriched category as compared with other groups, and green indicating depleted terms