Fig. 7.

Sodium arsenite induces SGs in HeLa cells. a SGs were visualized by staining classical marker of SGs: TIA-1 in untreated (control) and sodium arsenite-treated (0.6 mM; 60 min) (stress) cells. The cells were counter-stained with DAPI to visualize nuclei, scale bar = 10 μm. b, c Tau and p-tau are recruited into SGs. The cells were co-immuno-stained with primary antibodies specific for total-tau, p-tau and TIA-1. d The cells positive for SGs were calculated with FIJI software. More than 80% cells were identified positive for SGs after stress treatment. e Tau and p-tau-positive SGs per cell (average no. of SGs) were calculated using FIJI software. Significance was calculated by t-test, *p < 0.05. f–i Stress induced increase in tau-phosphorylation and TIA-1 levels. Representative immunoblots for TIA-1, total-tau and p-tau in control (untreated) and stress (arsenite treated) cells. Statistical significance was calculated with t-tests **p < 0.01, ***p < 0.001