FIGURE 2.

Relative quantification of the lactis and cremoris species and the biovar diacetylactis in reconstituted starters. Quantified proportions of lactis species and the biovar diacetylactis (IL1403 strain), and cremoris species (S72 strain), on the total Lactococcus population, in reconstituted starters with theoretical ratios of IL1403 to S72 of 5:95, 20:80, 50:50, 80:20, and 95:5. Data points represent ratios from three independently repeated experiments. P_lac: lactis species-specific probe; P_cre: cremoris species-specific probe; P_cit: biovar diacetylactis-specific probe; P_tot: L. lactis group-specific probe. Each P_lac/P_tot and P_cre/P_tot ratio was calculated from a duplex single well assay and P_cit/P_tot ratio was obtained from the values of two independent wells.