TABLE 2.

Primers/probe(s) sets targeting the gadB and citD genes of L. lactis and L. cremoris species.

Target gene	Target genotype	Primer or probe name	Sequence (5′–3′)	Amplicon or probe length (bp)
gadB		Forward F-gadB	TGGAAAATGAAATCATTCAACG	149
		Reverse R-gadB	ATATGTTTTATTTTCAGGTTCCTS
	lactis	Plac	FAM-TTATTTA+A+AGC+CTCA ATTGCTTCTTG-BHQ1	26
	cremoris	Pcre	FAM-TTATTTA+G+TGC+ATCAATTGCTTCTTG-BHQ1	26
	lactis and cremoris	Ptot	HEX-ACATAGTTAAA+T+G+CCATATTCATCC-BHQ1	25
citD		Forward F-citD	TGGTAAGCAAATCGAGGC	134
		Reverse R-citD	CACGTTCCACTACCGC
	biovar diacetylactis	Pcit	FAM-AGCTTATGCCATTGAAAATGCGGAC-BHQ1	25

HEX, hexachlorofluorescein; FAM, 6-carboxyfluorescein; BHQ, black hole quencher; +N in bold, locked nucleic acid, also corresponding to SNPs positions in P_lac and P_cre FAM-probes.