TABLE 5.
Plac/Ptot |
Pcre/Ptot |
Pcit/Plac |
||||||||
Condition | Time | n | Mean | SD | n | Mean | SD | n | Mean | SD |
Initial | ||||||||||
T0 | 6 | 0.75 | 0.047 | 6 | 0.28 | 0.042 | 6 | 0.21 | 0.039 | |
22°C | ||||||||||
T1 | 8 | 0.56 | 0.022 | 8 | 0.44 | 0.036 | 8 | 0.07 | 0.014 | |
T2 | 6 | 0.55 | 0.041 | 6 | 0.45 | 0.042 | 6 | 0.07 | 0.004 | |
35°C | ||||||||||
T1 | 9 | 0.77 | 0.007 | 9 | 0.22 | 0.01 | 9 | 0.06 | 0.011 | |
T2 | 6 | 0.53 | 0.043 | 6 | 0.48 | 0.043 | 6 | 0.24 | 0.051 |
Plac/Ptot, Pcre/Ptot and Pcit/Plac ratios were quantified at the start of the fermentation (T0) and when pH reached 4.5 (T1) and then 4.3 (T2) during growth at two different temperatures, 22°C and 35°C. Each Plac/Ptot and Pcre/Ptot ratio was calculated from a single well of a duplex assay and the Pcit/Plac ratio was obtained from values of two independent wells. The number of independently repeated experiments (n), the mean and standard deviation (SD) of the ratios are reported.