TABLE 1.
Xylem sap-specific exoproteins of V. longisporum.
| Name | Vl43 identifier | Best Hit V. dahliae JR2 | Protein domain | Peptide counts |
XyS/SXM | s/l ratio | ||
| SXM | XyS | |||||||
| Proteins acting on carbohydrates | Gla1 | vl43-au16.g17458.t1 | VDAG_JR2_Chr8g11020a | Glycoside hydrolase 15, | 0 | 8.2 | >8.2 | 0.76 |
| Gla1 | vl43-au16.g13024.t1 | VDAG_JR2_Chr8g11020a | Carbohydrate binding module family 20 | 0 | 3.8 | >3.8 | 0.62 | |
| Amy1 | vl43-au16.g6892.t1 | VDAG_JR2_Chr7g03330a | Glycoside hydrolase 13 | 1.5 | 12 | 8 | 0.68 | |
| vl43-au16.g9360.t1 | V. alfalfae: VDBG_05827 | Glycosyl hydrolases 134 | 0 | 7.5 | >7.5 | 0.77 | ||
| vl43-au16.g6746.t1 | VDAG_JR2_Chr3g11080a | Glycoside hydrolase 43 | 1.2 | 7.8 | 6.5 | 0.68 | ||
| vl43-au16.g12522.t1 | VDAG_JR2_Chr3g11080a | Glycoside hydrolase 43 | 1 | 5.8 | 5.8 | 0.61 | ||
| vl43-au16.g12596.t1 | VDAG_JR2_Chr6g04040a | Glycosyl hydrolases 11 | 0.8 | 5.2 | >5.2 | 0.55 | ||
| vl43-au16.g15027.t1 | VDAG_JR2_Chr6g09340a | Polysaccharide lyase 6 | 2.2 | 11.2 | 5.1 | 0.57 | ||
| vl43-au16.g5309.t1 | VDAG_JR2_Chr6g09340a | Polysaccharide lyase 6 | 2.3 | 10.8 | 4.7 | 0.59 | ||
| vl43-au16.g207.t1 | VDAG_JR2_Chr1g06940a | Glycoside hydrolase 16 | 2.2 | 9 | 4.1 | 0.56 | ||
| vl43-au16.g14986.t1 | VDAG_JR2_Chr1g06940a | Glycoside hydrolase 16 | 2.3 | 9.5 | 4.1 | 0.55 | ||
| vl43-au16.g15947.t1 | VDAG_JR2_Chr3g00250a | Glycoside hydrolase 131 | 1 | 4 | 4 | 0.43 | ||
| vl43-au16.g9097.t1 | V. alfalfae: VDBG_03110 | Glycoside hydrolase 12 | 1 | 2.8 | 2.8 | 0.38 | ||
| vl43-au16.g16765.t1 | VDAG_JR2_Chr1g04240a | Glycosyl transferase 1 | 0.3 | 1.7 | >1.7 | 0.49 | ||
| vl43-au16.g9025.t1 | VDAG_JR2_Chr7g01210a | Carbohydrate esterase 1, Carbohydrate binding module family 1 | 0.2 | 1.3 | >1.3 | 0.49 | ||
| Proteins with domains interacting with carbohydrates | Cbd1 | vl43-au16.g11636.t1 | VDAG_JR2_Chr4g04440a | Auxiliary activity 13, Carbohydrate binding module family 20 | 1.7 | 13.8 | 8.1 | 0.71 |
| Cbd1 | vl43-au16.g3945.t1 | VDAG_JR2_Chr4g04440a | 1.3 | 6.8 | 5.2 | 0.55 | ||
| vl43-au16.g5519.t1 | VDAG_JR2_Chr6g09790a | Auxiliary activity 9 | 2 | 8.2 | 4.1 | 0.51 | ||
| vl43-au16.g20506.t1 | VDAG_JR2_Chr6g09790a | Auxiliary activity 9 | 2.3 | 8.8 | 3.8 | 0.46 | ||
| vl43-au16.g11273.t1 | VDAG_JR2_Chr6g05220a | WSC Carbohydrate binding domain | 3.2 | 11.7 | 3.7 | 0.46 | ||
| Pathogenicity factors | Nlp3 | vl43-au16.g9727.t1 | VDAG_JR2_Chr4g05950a | Necrosis inducing protein | 0.3 | 8.3 | >8.3 | 0.84 |
| Nlp3 | vl43-au16.g96.t1 | VDAG_JR2_Chr4g05950a | Necrosis inducing protein | 0.2 | 6.3 | >6.3 | 0.81 | |
| Nlp2 | vl43-au16.g3884.t1 | VDAG_JR2_Chr2g05460a | Necrosis inducing protein | 1 | 4.8 | 4.8 | 0.61 | |
| Nlp2 | vl43-au16.g12566.t1 | VDAG_JR2_Chr2g05460a | Necrosis inducing protein | 1.8 | 6.5 | 3.6 | 0.47 | |
| Cp2 | vl43-au16.g16459.t1 | VDAG_JR2_Chr2g07000a | Cerato-platanin | 0.3 | 1.8 | >1.8 | 0.41 | |
| Peptidases | Mep2 | vl43-au16.g19470.t1 | VDAG_JR2_Chr1g21900a | Peptidase M43 | 1 | 8.8 | 8.8 | 0.57 |
| Mep2 | vl43-au16.g11262.t1 | VDAG_JR2_Chr1g21900a | Peptidase M43 | 1.3 | 8.7 | 6.7 | 0.55 | |
| Mep1 | vl43-au16.g19320.t1 | VDAG_JR2_Chr8g09760a | FTP domain, Peptidase M36, fungalysin | 1.7 | 14 | 8.2 | 0.69 | |
| Mep1 | vl43-au16.g14388.t1 | VDAG_JR2_Chr8g09760a | 1.8 | 13.5 | 7.5 | 0.65 | ||
| Proteins involved in redox processes | vl43-au16.g2684.t1 | VDAG_JR2_Chr8g10630a | FAD-binding domain | 6 | 13.2 | 2.2 | 0.33 | |
| vl43-au16.g13340.t1 | VDAG_JR2_Chr8g10630a | FAD-binding domain | 8 | 15.3 | 1.9 | 0.34 | ||
| Other | vl43-au16.g18734.t1 | VDAG_JR2_Chr1g04640a | Ubiquitin 3 binding protein But2, C-terminal | 9.7 | 18.8 | 1.9 | 0.37 | |
Vl43, V. longisporum 43; SXM, simulated xylem medium; XyS, xylem sap; s/l, signal-to-level; FTP, fungalysin/thermolysin propeptide; WSC, putative carbohydrate-binding domain.