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. 2020 Aug 12;10:13661. doi: 10.1038/s41598-020-70602-0

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Incorporation of GMPCPP-tubulin decreases microtubule depolymerization rate and increases frequency of interruptions in microtubule depolymerization. (a) Net length of microtubule lost during the time of experiments for a representative microtubule for each GMPCPP-tubulin incorporation examined: 0% (black), 40% (blue), 50% (green), 70% (yellow), 80% (red), and 100% (maroon). (b) The instantaneous loss rates over the time course of experiments for the representative microtubules corresponding to those in (a). (c) The average percentage of time microtubules were interrupted as a function of % GMPCPP-tubulin incorporation. Error bars are 95% confidence intervals. Data were fit using linear regression, y = 115.4x − 22.1, R² = 0.983.