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. 2020 Aug 12;10:13661. doi: 10.1038/s41598-020-70602-0

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Clusters of uncompressed dimers as a function of microtubule composition. (a) Histogram h(n) of cluster size distribution per 5 µm weighted by size n to make data at large n visible in plot. Histograms are for 10, 20, 30, 40, 50, and 60% (dark blue to light green) uncompressed microtubules. Uncompressed dimers were considered part of the same cluster if they share at least one vertical or horizontal subunit bond. (b, c) Heat maps of frequency of cluster footprints observed for 20% and 40% uncompressed, respectively, where footprints are given in terms of numbers of dimer rows and protofilaments spanned by the cluster. (d) Images at different rotations of the largest cluster observed for 20% uncompressed, comprising 21 dimers and having a footprint spanning 6 dimer rows and 11 protofilaments.