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. 2020 Aug 12;10:13585. doi: 10.1038/s41598-020-70239-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stability analysis of ANUBIX. (A) Fraction of KEGG pathways with Coefficient of variation (CV) below 2% for different number of iterations. (B) ANUBIX p-values are stable—their variance is low, and proportional to the magnitude of the p-value. A randomly chosen MSigDB gene set, DAIRKEE_CANCER_PRONE_RESPONSE_BPA, was run 100 times against KEGG pathways. Standard deviation of the log(p-value) is plotted against the mean-log(p-values) for each pathway. Software: R version 3.4.3 https://www.r-project.org/.