FIGURE 3.

At 7 d posttransplantation, islet allografts from recipient livers were stained with HE and immunostained for expression of CD4, CD8α, and insulin. (A) Representative images of islet allograft recipients treated with vehicle or with 0.1 or 0.3 mg/kg trametinib (Tra; magnification, ×400; scale bar: 100 μm). (B) The number of CD4⁺ and CD8⁺ cells in the recipient liver were counted and compared between vehicle and Tra groups. (C) The insulin-positive area was measured and compared between groups. Data are expressed as the mean ± SD of 2 independent experiments (n = 4/group) and compared using an unpaired Student’s t test (*P < 0.05). HE, hematoxylin and eosin; Tra, trametinib.