FIGURE 4.

CD4/CD8 and naive/effector memory T-cell subsets of the liver and spleen were analyzed by flow cytometry at d 7 posttransplantation in the presence of 0.1 or 0.3 mg/kg trametinib (Tra) or vehicle. (A) Percentages of CD4⁺ and CD8⁺ T cells and the CD8⁺/CD4⁺ ratio. (B) Percentage of naive (CD62L⁺CD44^–) and effector memory (CD62L^–CD44⁺) T cells among the CD4⁺ T-cell and (C) CD8⁺ T-cell populations. Data are representative of 3 independent experiments (mean ± SD; n = 3/group; 1-way ANOVA followed by the Tukey-Kramer test [*P < 0.05 vs vehicle, ** P < 0.01 vs vehicle]).