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. 2020 Aug 12;11:4034. doi: 10.1038/s41467-020-17626-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Targeted high-throughput sequencing of off-target sites in edited (RNP) or electroporated-only (Mock) HSPCs (n = 2 experiments from two different donors; no significant difference between RNP and Mock samples when pooled and analysed with two-tailed paired Student’s t test). b Sequences of off-target sites identified by GUIDE-seq for WAS gRNA. The intended target sequence is shown in the top line with mismatches to the on-target site shown and highlighted in colour. GUIDE-seq sequencing read counts are shown to the right of each site. GUIDE-seq sequencing read counts are shown to the right of each site. c Targeted high-throughput sequencing of off-target sites in edited (RNP) or electroporated-only (Mock) HSPCs (n = 2 experiments from two different donors; no significant difference between RNP and Mock samples when pooled and analysed with two-tailed paired Student’s t test). d Karyotype analysis of unmanipulated (Mock) and genome edited (RNP) HSPCs showed no chromosomal abnormalities (100 cells analysed for each condition). Source data are provided as a Source Data file.